Question
a. Given below is the stepwise schematic representation of the process of electrophoresis. Identify the alphabets representing (i) Anode end (ii) smallest/lightest DNA strand in the matrix (iii) Agarose gel
Image
b. What is elution? State the importance of elution in this process.

Answer

a. i. Anode = S
ii. Smallest/lightest DNA Strand = R
iii. Agarose gel = T
b. The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with cloning vectors.

Need a full question paper?

Generate a complete, print-ready paper with questions like this in minutes — across 16+ boards, with answer keys.

Start Generating Free

Similar questions

Among the ecosystem services are control of floods and soil erosion. How is this achieved by the biotic components of the ecosystem?
How would you find genotype of a tall pea plant bearing white flowers? Explain with the help of a cross. Name the type of cross you would use.
How many types of restriction endonucleases are found. Why they are called as molecular scissors?
With respect to Messelson and Stahl’s Experiment, answer the following questions:
  1. Identify the method used to distinguish between heavy and light isotopes of nitrogen.
  2. With the help of diagrams, compare the results for the DNA isolated after 20 minutes of experiment with the DNA which was isolated after 40 minutes.
Define pollination. Explain any two contrivances of self-pollination.
Study the table showing the population interaction between species Z and Y respectively. Assign the appropriate +/– signs for A,B,D,E and respective interactions for C and F.
Image
Make a list of any three outbreeding devices that flowering plants have developed and explain how they help to encourage cross-pollination.
  1. Why are grasshopper and Drosophila said to show male heterogamity? Explain.
  2. Explain female heterogamity with the help of an example.
A person has been diagnosed to be HIV positive.
  1. Name the test which the person underwent.
  2. Write the full name of the pathogen involved and describe its structure.
  3. Which particular cells of this person are likely to get destroyed?
Mention the name of the causal organism, symptoms and the mode of transmission of the disease Amoebiasis.