$(1)$ Denaturation of proteins causes loss of secondary and tertiary structures of the protein.
$(2)$ Denaturation leads to the conversion of double strand of $DNA$ into single strand.
$(3)$ Denaturation affects primary structure which gets distorted.
(a) Water solubility is maximum at a $pH$ when concentrations of anions and cations are equal
(b) They give ninhydrin test
(c) On reacting with nitrous acid give off ${N_2}$
