MCQ
At which temperature a $\text{DNA}$ molecule is denatured by heat in $\text{PCR}:$
- A$ 70^{\circ}-80^{\circ} \mathrm{C} $
- ✓$ 90^{\circ}-95^{\circ} \mathrm{C} $
- C$ 42^{\circ} \mathrm{C} $
- D$ 50^{\circ}-65^{\circ} \mathrm{C} $
✓
Answer
Correct option: B.
$ 90^{\circ}-95^{\circ} \mathrm{C} $
Denaturation$:$ The reaction temperature is increased to $95$ degree centigrade, which melts $($disrupts the hydrogen bond between complementary bases$)$ all $\text{dsDNA}$ into single$-$stranded $\text{DNA (ssDNA)}.$
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