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$I.$ Polymerase Chain Reaction $(PCR)$ is a technique of synthesizing multiple copies of the desired gene in vitro
$II.$ This technique was developed by Kary Mullis in $1985$
$III.$ A single $PCR$ amplification cycle involves three basic steps; denaturation, annealing and extension
Which of the statement given above are correct?
$(A)$ Colostrum is recommended for the new born because it is rich in antibody.
$(B)$ Chikungunya is caused by a Gram negative bacterium.
$(C)$ Tissue culture has proved useful in obtaining virusfree plants.
$(D)$ Beer is manufactured by distillation of fermented grape juice. How many of the above statements are wrong?
$i. r-RNA$ provides the template for synthesis of proteins.
$ii.$$ t-RNA$ brings amino acids and reads the genetic code.
$iii. $$RNA$ polymerase binds to promoter and initiates transcription.
$iv. $A segment of $DNA$ coding for polypeptide is called intron.