Question
Describe the steps in producing somatic hybrids from protoplast.
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Answer
It is carried out by the following steps:
- Single cells from selected plants are isolated.
- The cell walls of cells are digested by enzymes like pectinase and cellulase, to expose the naked protoplasts.
- Naked protoplasts surrounded only by plasma membranes are isolated.
- The isolated protoplasts are fused to obtain hybrid protoplasts under sterile conditions in special nutrient media.
- The hybrid protoplasts are cultured in a suitable media to form new plant.
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