- AJoining bits of $DNA$
- ✓Splitting or separation of two strands of $DNA$
- CRenaturation
- DDenaturation
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$(i)$ It is a membrane bound space found in the cytoplasm.
$(ii)$ It is bound by a single membrane called tonoplast.
$(iii)$ It contains water, sap, excretory products and other materials not useful to the cell.
$(iv)$ It has higher concentration of sap than the cytoplasm.
$I.$ Recombinant $DNA$ technology popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic material by man in vitro
$II. pBR322$ is the first artificial cloning vector developed in $1977$ by Boliver and Rodriquez from $E$. coli plasmid
$III.$ Restriction enzymes belong to a class of enzymes called nucleases
Which of the statements given above are correct?