STD 12 - 5. molecular basis of inheritance — BIOLOGY STD 12 Science — Question

$I.$ Extraction of the desired gene product

$II.$ Amplification of the gene of interest

$III.$ Isolation of a desired $DNA$ fragment

$IV.$ Ligation of the $DNA$ fragment into a vector

$V.$ Insertion of recombinant $DNA$ into the host

Correct order is

$(I)$ Separation of $DNA$ fragment by electrophoresis.

$(II)$ Digestion of $DNA$ by restriction endonucleases.

$(III)$ Hybridization using labeled $VNTR$ probe

$(IV)$ Isolation of $DNA$.

$(V)$ Detection of hybridized $DNA$ fragments by autoradiography.

$(VI)$ Transferring of separated $DNA$ fragment to synthetic membranes.

$A.$ Administration of progestogens
$B.$ Progestogen-estrogen combination
$C.$ $IUDs$ inserted within $72$ hours of coitus