Question
Electrophoresis.

Answer

  1. Electrophoresis is a technique that involves migration and separation of charged particles under the influence of electric field.
  2. It is used for the separation of charged molecules like DNA, RNA and proteins, by application of an electric field.
  3. Movement of charged particles is determined by particle size, shape and charge.
  4. DNA is negatively charged and hence it moves towards the positive anode.
  5. It results in size separation of DNA fragments as small fragments of DNA molecules move faster.
  6. Different types of electrophoresis are agarose gel electrophoresis, PAGE and SDA PAGE.

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