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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Bihar BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes2 Marks
Question
Explain briefly:
PCR
✓

Answer

PCR = Polymerase chain reaction (in vitro method) is a molecular biological technique for enzymatically replicating DNA without using a living organism, such as E. coli or yeast.

3 steps in PCR are-

  1. Denaturation of desired double strand DNA-to ssDNA
  2. Annealing of primer to ssDNA (single standard).
  3. Extension of primer by Taq DNA polymerase isolated form Thermits aquaticus.

Uses- Amplification of desired gene/ gene cloning.

Advantage- More output, greater efficiency, less error prone, less human interference and cyclic and automated.

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