Question
- Explain how to find whether an E.coli bacterium has transformed or not, when a recombinant DNA bearing ampicillin-resistant gene is transferred into it.
- What does the ampicillin-resistant gene act as, in the above case?
✓
Answer
- The recombinant/ transformant can be selected out from the non-recombinants/ non-transformants by plating the transformants on ampicillin-containing medium. The transformants will grow in it, while the non-transformants will not grow.
- It acts as a selectable marker.
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