Question
Explain the basis on which the gel electrophoresis technique works. Write any two ways the products obtained through this technique can be utilised.
✓
Answer
- In gel electrophoresis, the molecules resolve according to their molecular size by the sieving effect provided by the gel.
- DNA being negatively charged, moves towards the anode in the electric field.
- Use of DNA isolated:
- The DNA fragments may be used to construct recombinant DNA by joining them with cloning vectors.
- The desired DNA fragments may be amplified, i.e. making multiple copies, by polymerase chain reaction (PCR).
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