Molecular Basis of Inheritance — BIOLOGY STD 12 Science — Question

1. Write an equation for Verhaulst-Pearl Logistic Growth, where.

N - Population density at a timet E.

r - Intrinsic rate of natural increase.

K - Carrying Capacity.

2. Draw a graph for a population whose population density has reached the carrying capacity.
3. Why is this logistic growth model considered a more realistic one for most animal populations?
4. Draw a growth curve where resources are not limiting to growth of a population.

Explain the process of replication of a retrovirus after it gains entry into human body.

Explain the process of divergent evolution in detail. Also, write down the driving force behind it.

Why do some adolescents start taking drugs? How can the situation be avoided?

A pregnant woman was adviced to undergo medical termination of pregnancy. Her doctor found that the foetus in her womb was developed from a zygote made of an XX egg fertilized by a Y-bearing sperm? Why was she advised to undergo MTP?

A youth in his twenties met with an accident and succumbed to the injuries. His parents agreed to donate his organs. So, their:

1. List any two essential clinical steps to be undertaken before any organ transplant.
2. Why is the transplant rejected sometimes?
3. What views would you share with your health club members to promote organ donation?

Read the following and answer any four questions from (i) to (v) given below:
Crown gall is a neoplastic disease of most dicotyledonous plants and is caused by the soil bacterium Agrobacterium tumefaciens. A large extra chromosomal plasmid in these bacteria was found to be responsible for this disease. The plasmid is known as Ti plasmid. Bacteria free crown gall cells can be cultured in the absence of phytohormones. Ti plasmid is widely used in genetic engineering to deliver the desirable genes. The part of Ti plasmid transferred into plant cell DNA is called T-DNA. T-DNA with desired DNA segment is inserted into the chromosome of the host plant where it produces copies of itself.

1. Which of the following is the full full of T-DNA?

1. Transfer DNA.
2. Tumour inducing DNA.
3. Transgenic DNA.
4. None of these.

2. Ti plasmid cannot infect and develop crown gall in,

1. Tomato.
2. Maize.
3. Soybean.
4. sunflower.

3. While making transgenic plant, T-DNA is inserted into the host cell, it is then:

1. Integrated into the target host genome.
2. Lie independent of the host genome.
3. Rupture the host cell wall.
4. Produce oncogenic factors in the host.

4. Agrobacterium mediated gene transfer in plants:

1. Allows relatively large segment of DNA.
2. Transfer of DNA with defined ends and minimal rearrangement.
3. High quality and fertility in transgenic plants.
4. All of these.

5. Assertion: In Agrobacterium mediated gene transfer in plants, transgenic plants do not develop tumors.

Reason: In T-DNA tumor producing genes are deleted during the process of gene transfer.

1. Both assertion and reason are true and reason is the correct explanation of assertion.
2. Both assertion and reason are true but reason is not the correct explanation of assertion.
3. Assertion is true but reason is false.
4. Both assertion and reason are false.

Water logging and soil salinity are some of the problems that have come in the wake of the Green Revolution. Discuss their causes and adverse effects to the environment.

Read the following and answer any four questions from (i) to (v) given below:
Insulin used to cure diabetes was earlier extracted from pancreas of slaughtered cattle and pigs. Insulin extracted from an animal source, though caused some patients to develop allergy or other types of reactions to the foreign protein. Human insulin consists of two short polypeptide chains : chain A and chain B, that are linked together by disulphide bridges. In mammals including humans, insulin is synthesised as a pro-hormone which contains an extra stretch called the C-peptide. This C peptide is not present in mature insulin and is removed during maturation into insulin.

1. Identify A in the given figure.

1. Polypeptide chain A.
2. Polypeptide chain B.
3. Polypeptide chain C.
4. None of these.

2. The following is a list of some stages involved in producing human insulin from genetically engineered bacteria.

1. The bacteria are cultured in a fermenter for large scale production.
2. Recombinant insulin is extracted from the bacterial cells that expresses insulin gene.
3. The same restriction enzyme is used again to cut the bacterial plasmid for insertion of the human insulin gene.
4. Bacteria take up the plasmid carrying the insulin gene.
5. A restriction enzyme is used to cut human DNA to extract the insulin gene.

Select the correct order of these stages.

1. 1, 5, 3, 4, 2
2. 2, 4, 3, 5, 1
3. 4, 5, 3, 2, 1
4. 5, 3, 4, 1, 2

3. To insert the insulin gene into bacterial DNA, both the bacterial plasmid and the human chromosome containing the insulin gene are treated with the same restriction enzyme. Using the same restriction enzyme ensures that

1. DNA ligase is able to join the segments of human and bacterial DNA.
2. The exact length of nucleotides matching the insulin gene is removed from the plasmid.
3. Both the bacterial and human DNA will contain sticky ends.
4. Sticky ends in the cut plasmid and insulin gene are complementary.

4. Why is the fermentor important for the production of human insulin by transgenic bacteria?

1. It provides optimal conditions for the transgenic to multiply rapidly.
2. It facilitates the extraction and purification of insulin from the transgenic bacteria.
3. It maximise the rate of fermentation of the transgenic bacteria.
4. It provides the low-oxygen conditions that are important for insulin production.

5. A bacteriologist carries out his first attempt at engineering E.coli with the gene for human insulin. During the process, he realises that his stock of DNA ligase has depleted but decides to continue anyway. What is a likely consequence of his decision?

1. Bacteria with the rDNA will not be able to form colonies in a fermenter.
2. The resulting plasmids are not able to enter the E.coli bacteria even after applying heat shock.
3. The resulting E.coli bacteria do not contain the human insulin gene.
4. The bacterial plasmids do not have sticky ends and are unable to accommodate the human gene.

Answer the following questions with respect to recombinant DNA technology:
i. Why is plasmid considered to be an important tool in rDNA technology? From where can plasmids be isolated? (Any two sources)
ii. Explain the role of ori and selectable marker in a cloning vector.
iii. r-DNA technology cannot proceed without restriction endonuclease. Justify.