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Molecular Basis of Inheritance — BIOLOGY STD 12 Science — Question

Bihar BoardEnglish MediumSTD 12 ScienceBIOLOGYMolecular Basis of Inheritance4 Marks
Question
Explain with diagram - Hershey and Chase experiment.###On the basis of experimental proof explain the diagram that DNA is genetic material.###How did Hershey and Chase differentiate between DNA and protein in their experiment while proving that DNA is the genetic material?
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Answer

→ The unequivocal proof that DNA is the genetic material came from the experiments of Alfred Hershey and Martha Chase (1952). They worked with viruses that infect bacteria called bacteriophages.
→ The bacteriophage attaches to the bacteria and its genetic material then enters the bacterial cell.
→ The bacterial cell treats the viral genetic material as if it was its own and subsequently manufactures more virus particles.
→ Hershey and Chase worked to discover whether it was protein or DNA from the viruses that entered the bacteria.
→ They grew some viruses on a medium that contained radioactive phosphorus and some others on medium that contained radioactive sulfur.
→ Viruses grown in the presence of radioactive phosphorus contained radioactive DNA but not radioactive protein because DNA contains phosphorus but protein does not.
→ Similarly, viruses grown on radioactive sulfur contained radioactive protein but not radioactive DNA because DNA does not contain sulfur.
→ Radioactive phages were allowed to attach to E.coli bacteria.
→ Then, as the infection proceeded, the viral coats were removed from the bacteria by agitating them in a blender.
→ The virus particles were separated from the bacteria by spinning them in a centrifuge.
→ Bacteria which was infected with viruses that had radioactive DNA were radioactive, indicating that DNA was the material that passed from the virus to the bacteria.
→ Bacteria that were infected with viruses that had radioactive proteins were not radioactive.
→ This indicates that proteins did not enter the bacteria from the viruses.
→ DNA is therefore the genetic material that is passed from virus to bacteria.
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