- ALag phase
- ✓Log phase
- CPlateau stage
- DFirst stage
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$I.$ In microinjection method foreign $DNA$ is directly injected into the nucleus of animal cell or plant cell by using micro needles or micro pipettes
$II.$ Microinjection method is used in oocytes, eggs and embryo
$III.$ Electroporation is the formation of temporary pores in the plasma membrane of host cell by using lysozyme or calcium chloride
$IV.$ In chemical mediated gene transfer method certain chemicals such as $\mathrm{CO}_{2}$ help foreign $DNA$ to enter the host cell
Which of the statements given above are correct?

$(i)$ Contractile vacuole takes part in osmoregulation and excretion.
$(ii)$ Food vacuole is formed by engulfing the food particles.
$(iii)$ The vacuole is bound by a double membrane called tonoplast.
$(iv)$ Vacuole can occupy upto $90$ percent of the volume of the cell.