MCQ
Function of restriction endonuclease enzyme is :
  • A
    Useful in genetic engineering.
  • B
    Protects the bacterial $\text{DNA}$ against foreign $\text{DNA}$.
  • Both $A$ and $B$
  • D
    Helpful in protein synthesis.

Answer

Correct option: C.
Both $A$ and $B$
Restriction systems identify the origin of incoming $\text{DNA}$ and destroy it
if recognized as foreign. Restriction endonucleases recognize specific
sequences in the incoming $\text{DNA}$ and digest the $\text{DNA}$ into fragments.
They
cut the $\text{DNA}$ either at specific sites or more randomly.
Hence, restriction endonucleases are synthesized by bacteria as part of their defense mechanism.
These enzymes are used in genetic engineering to cut the gene of the interest from source $\text{DNA}$ and to introduce them into the vectors;
but this is not a natural function of restriction endonucleases.
Transcription is the process of synthesis of $\text{RNA}$ using the $\text{DNA}$ template.
$\text{RNA}$ polymerase perform this function by adding ribonucleotides to the $\text{RNA}$ primer.
Translation of nucleotide sequence of $\text{mRNA}$ into amino acid sequence of protein is called as protein synthesis.
It is performed by $\text{tRNA}$.
Restriction enzymes do not play any role in these two processes.

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