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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Maharashtra BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes3 Marks
Question
  1. How are recombinant vectors created?
  2. For creating one recombinant vector only one type of restriction endonuclease is required. Give reason.
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Answer

  1. The vector DNA is cut at a particular restriction site using a restriction enzyme (to cut the desired DNA segment). The alien DNA is then linked with the plasmid DNA using an enzyme called ligase to form the recombinant vector.
  2. A restriction enzyme recognizes and cuts the DNA at a particular sequence, called recognition site. If more than one restriction enzymes are present, they will generate several segments and will complicate gene cloning.

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