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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Maharashtra BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes1 Mark
MCQ
In a genetic engineering experiment, restriciton enzymes can be used for :
  • A
    Bacterial $\text{DNA}$ only
  • B
    Viral $\text{DNA}$ only
  • ✓
    Any $\text{DNA}$ fragment
  • D
    Eukaryotic $\text{DNA}$ only
✓

Answer

Correct option: C.
Any $\text{DNA}$ fragment
Restriction enzymes, also called restriction endonucleases, bind to $\text{DNA}$ and cleave the double strand, forming smaller pieces of $\text{DNA}$.
There are three types of restriction enzymes;
Type $\text{I}$ restriction enzymes recognize a $\text{DNA}$ sequence and cut the strand randomly more than one thousand base pairs away from the site.
Type $\text{II}$ restriction enzymes, the most useful for molecular biology laboratories, recognize and cut the $\text{DNA}$ strand predictably at a specific sequence which is usually less than ten base pairs long.
Type $\text{III}$ restriction enzymes are similar to
​​​​​​​Type $\text{I},$ but these cut the $\text{DNA}$ about thirty base pairs from the recognition sequence.

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