- At the time of fertilization of ovum
Explanation:
In humans, sex is determined by the father.
The mother produces only one kind of gamete - having X-chromosome only.
The father produces two kinds of gametes - where one contains the X chromosome and another one contains the Y chromosome.
During fertilization, if the X chromosome of the father fuses with the ovum of the mother, which contains an X chromosome, it becomes a girl child.If the Y chromosome of the father fuses with the ovum containing X in the mother, it results in a boy.