Isoelectric point is a
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\begin{matrix}
C{{H}_{2}}OOCR' \\
|\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\, \\
\end{matrix}\,\,\, \\
CHOOCR\,\,\,\,\,\,\, \\
|\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\, \\
C{{H}_{2}}OOCR''' \\
\end{matrix}$ $\underset{\text{Hydrolysis}}{\mathop{\xrightarrow{\text{Enzyme}}}}$ $\begin{matrix}
\begin{matrix}
C{{H}_{2}}OH \\
|\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\, \\
\end{matrix} \\
CHOH\,\,\, \\
|\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\, \\
C{{H}_{2}}OH \\
\end{matrix}$ $+\begin{matrix}
\begin{matrix}
R'COOH \\
+ \\
\end{matrix} \\
R''COOH \\
+ \\
R'''COOH \\
\end{matrix}$The enzyme used in the above reaction is
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