Question
List in proper sequence the processes involved in recombinant DNA (rDNA) technology.
✓
Answer
The steps are:
- Isolation of DNA.
- Fragmentation of DNA by restriction endonucleases.
- Isolation of a desired DNA fragment.
- Amplification of the desired DNA fragment.
- Ligation of the DNA fragment into a vector.
- Transferring the recombinant DNA into the host.
- Culturing the host cells and extraction of the desired gene product on a large scale.
- Downstream processing, i.e. separation and purification.
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