STD 12 - 9. biotechnology principlas and process — NEET STD 11 Science — Question

$I.$ $O_{2}$ helps in releasing metabolic energy, which is essential for growth

$II.$ Nutrients are required by plants for the synthesis of protoplasm

$III.$ Change in temperature could be the detrimental for the survival of an organism

$IV$. Light and gravity don't affect the stages of growth

Choose the correct option

$I.$ Polymerase Chain Reaction $(PCR)$ is a technique of synthesizing multiple copies of the desired gene in vitro

$II.$ This technique was developed by Kary Mullis in $1985$

$III.$ A single $PCR$ amplification cycle involves three basic steps; denaturation, annealing and extension

Which of the statement given above are correct?