Question
Name two microbes that are useful in biotechnology experiments.

Answer

  • Agrobacterium tumefaciens.
  • Escherichia coli.
  • Bacillus thuringiensis.

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Golden rice was engineered from normal rice by Potrykus and Beyer in the 1990s. The typical golden colour is due to the production of $\beta$-carotene a precursor of vitamin-A. Golden rice differs from its parental strain by the addition of three $\beta$-carotene genes. These included two genes from daffodil plant and third from a bacterium. The incorporation of these genes allows the rice plant to modify certain metabolic pathways in its cells to produce $\beta$-carotene.
  1. Due to genetic modification golden rice plants produce and store $\beta$-carotene in:
  1. Stem.
  2. Seed.
  3. Leaves.
  4. All of these.
  1. Transfer of genes to produce golden rice is achieved by:
  1. Agrobacterium.
  2. pBR322
  3. $\lambda$-phage.
  4. gene gun.
  1. In golden rice two genes were taken from:
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  2. Erwinia.
  3. Coryza saliva.
  4. None of these.
  1. Golden rice is helpful to fight against disease caused by the deficiency of:
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  2. Vitamin C
  3. Vitamin A
  4. Vitamin D
  1. Golden rice was genetically engineered by
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  3. Banting and Best.
  4. Kohler and Milstein.
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