STD 12 - 9. biotechnology principlas and process — Biology STD 12 Science — Question

$(I)$ Use of bio resources by multinational companies and organization without proper authorization from the country and people concerned compensatory payment.

$(II)$ Traditional knowledge related to bio-resources can be exploited of Their bio-resources.

$(III)$ Nations are developing laws to prevent the unauthorized exploitation of Their bio-resources.

$I.$ Extraction of the desired gene product

$II.$ Amplification of the gene of interest

$III.$ Isolation of a desired $DNA$ fragment

$IV.$ Ligation of the $DNA$ fragment into a vector

$V.$ Insertion of recombinant $DNA$ into the host

Correct order is