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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Maharashtra BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes1 Mark
MCQ
Restriction endonucleases are used as :
  • A
    Molecular build up at nucleotides
  • B
    Molecular degradation to break up a $\text{DNA}$
  • ✓
    Molecular knives for cutting $\text{DNA}$ at specific sites
  • D
    Molecular cement to combine $\text{DNA}$ sites
✓

Answer

Correct option: C.
Molecular knives for cutting $\text{DNA}$ at specific sites
Restriction endonucleases are enzymes that cleave the sugar $-$ phosphate backbone of $\text{DNA}$.
In most practical settings, a given enzyme cuts both strands of duplex $\text{DNA}$ within a stretch of just a few bases.
Several thousand different restriction endonucleases have been isolated, which collectively exhibit a few hundred different sequence $($substrate$)$ specificities.

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