Question
Restriction enzymes are found in bacteria (and other prokaryotes). They recognize and bind to specific sequences of DNA, called restriction sites. Each restriction enzyme recognizes just one or a few restriction sites.
When it finds its target sequence, a restriction enzyme will make a double-stranded cut in the DNA molecule.
i. Write the palindromic nucleotide sequence for the following DNA segment.
5'-GAATTC-3'
ii. Name the restriction endonuclease that recognises this sequence.
iii. How are sticky ends produced? Mention their role.
When it finds its target sequence, a restriction enzyme will make a double-stranded cut in the DNA molecule.
i. Write the palindromic nucleotide sequence for the following DNA segment.
5'-GAATTC-3'
ii. Name the restriction endonuclease that recognises this sequence.
iii. How are sticky ends produced? Mention their role.
