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STD 12 - 5. molecular basis of inheritance — Biology STD 12 Science — Question

CBSE BoardEnglish MediumSTD 12 ScienceBiologySTD 12 - 5. molecular basis of inheritance1 Mark
MCQ
The commonly used $DNA$ fingerprinting technique in forensic studies is simply a method involving
  • ✓
    Southern blotting
  • B
    Northern blotting
  • C
    Eastern blotting
  • D
    Western blotting
✓

Answer

Correct option: A.
Southern blotting
a
A technique developed by $EM$ Southern in $1975$ for detection of a specific $DNA$ sequences (gene or other) in a large, complex sample of $DNA$ $(e . g .$, cellular $DNA$). It is also used to determine the molecular weight of a restriction fragment and to measure relative amounts in different sample. Uses Southern blots are used in gene discovery and mapping, evolution and development studies, diagnostics and forensics.In regards to genetically modified organisms, Southern blotting is used as a definitive test to ensure that a particular section of $DNA$ of known genetic sequence has been successfully incorporated into the genome of the host organism

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