STD 11 - 8. Cell the unit of life — NEET STD 11 Science — Question

$I$. causes partial oxidation of glucose (one molecule) to form $2$-molecules of pyruvic acid and $2$ $ATP$ as net gain

$II$. takes place in all living cells

$III$. uses $2$ $ATP$ at two steps

$IV$. scheme was given by Gustav Embden, Otto Mayerhof and J Parnas

Choose the correct option containing appropriate statements from the above

$I.$ Extraction of the desired gene product

$II.$ Amplification of the gene of interest

$III.$ Isolation of a desired $DNA$ fragment

$IV.$ Ligation of the $DNA$ fragment into a vector

$V.$ Insertion of recombinant $DNA$ into the host

Correct order is