MCQ
The enzyme involved in transcription is:
- ARNA polymerase
- BDNA polymerase I
- CDNA polymerase II
- DDNA polymerase III
Explanation:
During transcription, RNA polymerase makes a copy of a gene from the DNA to mRNA as needed. During transcription, the DNA site at which RNA polymerase binds is called as a promoter. Transcription commences with one or more sigma factor protein binds to the RNA polymerase holoenzyme, allowing it to bind to promoter DNA. RNA polymerase creates a transcription bubble, which separates the two strands of the DNA helix. This is done by breaking the hydrogen bonds between complementary DNA nucleotides.
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|
$I$ |
$II$ |
$III$ |
|
$(a)$ PCR |
$(i)$ Specific base sequence |
$p.$ Taq polymerase |
|
$(b)$ Downstream processing |
$(ii)$ Inheritable gene |
$q.$ Ampicillin resistant |
|
$(c)$ Restriction endonucleasel |
$(iii)$ Amplification of $DNA$ |
$r.$ E. coli |
|
|
$(iv)$ Product separation and modification |
$s.$ Quality control |