MCQ
The stain used to visualise mitochondria is:
- AFast green.
- BSafranin.
- CAcetocarmin.
- DJanus green.
Explanation:
Janus green is used to stain mitochondria. Janus green act as an indicator and changes colour according to the amount of oxygen present. It oxidizes to blue colour in. presence of oxygen and in its absence changes its colour to pink. Safranin is used to stain nuclei and lignified walls of cell. Acetocarmine is used for staining nucleic acid and chromosomes. Fast green It is used for staining his tones at alkaline pH after acid extraction from DNA.
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$\begin{array}{|l|l|} \hline Column\;-I & Column\;-II \\ \hline A.\;Micro\;filament & i.\;Glycokalyx \\ \hline B.\;Micro\;tubule & ii.\;Actin \\ \hline C.\;Flagella & iii.\;Tubulin \\ \hline D.\;Outer\;layer\;of\;bacteria & iv.\;Outer\;compartment \\ \hline \end{array}$
$I$. causes partial oxidation of glucose (one molecule) to form $2$-molecules of pyruvic acid and $2$ $ATP$ as net gain
$II$. takes place in all living cells
$III$. uses $2$ $ATP$ at two steps
$IV$. scheme was given by Gustav Embden, Otto Mayerhof and J Parnas
Choose the correct option containing appropriate statements from the above