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STD 12 - 5. molecular basis of inheritance — BIOLOGY STD 12 Science — Question

Rajasthan BoardEnglish MediumSTD 12 ScienceBIOLOGYSTD 12 - 5. molecular basis of inheritance1 Mark
MCQ
What is the first step in the Southern blot technique ?
  • A
    Denaturation of $DNA$ on the gel for hybridization with specific probe
  • B
    Production of a group of genetically identical cells
  • ✓
    Digestion of $DNA$ by restriction enzyme
  • D
    Denaturation of $DNA$ from a nucleated cell such as the one from the scene of crime
✓

Answer

Correct option: C.
Digestion of $DNA$ by restriction enzyme
c
The Southern blot is used to detect and identify certain $DNA$ sequences in a sample of bodily fluid. It uses single-stranded $DNA$ to search out their complementary strands. When a Southern blot is performed on $DNA$, the first step is digestion of $DNA$ with restriction enzymes. Restriction enzymes cut $DNA$ at known sequences, and produces $DNA$ fragments of a certain length. Once the $DNA$ is cut into pieces, scientists conduct electrophoresis to separate them by size.

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