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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Rajasthan BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes1 Mark
MCQ
What is true about DNA polymerase used in PCR?
  • A
    It is used to ligate introduced DNA in recipient cells.
  • B
    It serves as selectable marker.
  • C
    It is isolated from a virus.
  • D
    It is active at high temperature.
✓

Answer

  1. It is active at high temperature.

Explanation:

DNA polymerase enzyme is an essential component for PCR due to its key role in synthesizing new DNA strands. Taq DNA polymerase ( synthesized from the thermophilic bacteria Thermus aquaticus) is the most common enzyme used for PCR amplification. This enzyme is extremely heat resistant with a half-life of 40 minutes at 95°C.

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