Question
Where is an 'operator' located in a prokaryote DNA? How does an operator regulate gene expression at transcriptional level in a prokaryote? Explain.
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Answer
The operator region is located adjacent to promoter elements/prior to structural gene. In regulation of gene expression.
- Switch off- The repressor binds to the operator region, & prevents transcription.
- Switch on- In the presence of inducer the repressor is inactivated, (by the interaction with the inducer) and operator allows RNA polymerase access to the promoter, & transcription proceeds.
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