$(A)$ Denaturation of proteins causes loss of secondary and tertiary structures of the protein
$(B)$ Denturation leads to the conversion of double strand of $DNA$ into single strand
$(C)$ Denaturation affects primary structure which gets distorted
$\begin{matrix}
\underset{X}{\mathop{H}}{{O}_{2}}C-C{{H}_{2}}-CH-C{{O}_{2}}\underset{Z}{\mathop{H}}\, \\
\,\,\,\,\,\,\,\,\,\,\,| \\
\,\,\,\,\,\,\,\,\,\,\,\,\,+N{{H}_{3}} \\
\end{matrix}$
The isoelectric point for the amino acid is