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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Maharashtra BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes2 Marks
Question
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
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Answer

If denaturation is missed then primers will not be able to join at the template. This will result in no extension, no amplification and thus a large number of copies of DNA cannot be made.

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