Question
Why are Okazaki fragments formed on lagging strand only?
✓
Answer
- The lagging template is the template strand with free 5’ end.
- The replication always starts at C-3 end of template strand and proceeds towards C-5 end.
- Both the strands of the parental DNA are antiparallel and new strands are always formed in 5′ → 3′ direction, i.e. DNA polymerase synthesizes new strand in only one direction i.e. 5′ → 3′ direction.
- Hence, the lagging templates becomes available for replication only discontinuously in small patches.
- The new lagging strand develops discontinuously away from the replicating fork in the form of small Okazaki fragments.
- Hence, Okazaki fragments formed on lagging strand only.
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