Download App

Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Bihar BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes3 Marks
Question
Why does the 'insertional inactivation' method to detect recombinant DNA is preferred to 'antibiotic resistance' procedure?
✓

Answer

The presence of a chromogenic substrate gives blue coloured colonies, in absence of an insert/in non-transformants, presence of an insert (in the enzyme site), results into (insertional inactivation of the β-galactosidase) colonies which do not produce colour.
Antibiotic resistance method requires duplicate plating/cumbersome procedure.

Need a full question paper?

Generate a complete, print-ready paper with questions like this in minutes — across 16+ boards, with answer keys.

Start Generating Free

Explore more

More "3 Marks Question" from Biotechnology: Principles and Processes→Biotechnology: Principles and Processes — all question types→BIOLOGY STD 12 Science question papers→Bihar Board STD 12 Science question papers→Bihar Board question paper generator→

Similar questions

F1 progeny of pea plant bearing violet flowers and snapdragon plan bearing red flowers were selfed to produce their respective F2 progeny. Compare the phenotypes, the genotypes and the pattern of inheritance of their respective F2 progeny.
→
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
  1. Any two restriction sites.
  2. Ori and rop genes.
  3. An antibiotic resistant gene.
→
What are selectable markers? Give examples.
→
  1. Write the importance of measuring the size of a population in a habitat or an ecosystem.
  2. Explain with the help of an example how the percentage cover is a more meaningful measure of population size than more numbers.
→
State the function of a reservoir in a nutrient cycle. Explain the simplified model of carbon cycle in nature.
→
A team of students are preparing to participate in the interschool sports meet. During a practice session, you find some vials with labels of certain cannabinoids.
  1. Will you report to the authorities? Why?
  2. Name a plant from which such chemicals are obtained.
  3. Write the effect of these chemicals on human body.
→
Explain with the help of an example the relationship between restriction endonuclease and a palindromic nucleotide sequence.
→
Draw a labelled sketch of a mature 7-celled, 8-nucleate embryo-sac.
→

Mention three physical properties and three chemical properties of soil, that determine the type of vegetation in a given region.

→
The sacred groves of Aravalli Hills and Ooty botanical garden, both aim at biodiversity conservation. How do they differ in their approaches? Explain.
→