Download App

Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Bihar BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes3 Marks
Question
  1. Why must bacterial cells be first made 'competent' in rDNA technology? How is the process carried out?
  2. Name the methods by which an alien DNA can be made to enter:
  1. A plant cell.
  2. An animal cell.
✓

Answer

  1.  
  1. DNA is a hydrophilic molecule that cannot pass through cell membrane; so the bacterial cells must be made competent to take up the DNA.
  2. The bacterial cells are made competent by treating them with a specific concentration of a divalent cation, such as calcium; this increases the efficiency with which DNA enters the bacterium.
  1.  
  1. Biolistics/ Gene gun.
  2. Micro-injection.

Need a full question paper?

Generate a complete, print-ready paper with questions like this in minutes — across 16+ boards, with answer keys.

Start Generating Free

Explore more

More "3 Marks Question" from Biotechnology: Principles and Processes→Biotechnology: Principles and Processes — all question types→BIOLOGY STD 12 Science question papers→Bihar Board STD 12 Science question papers→Bihar Board question paper generator→

Similar questions

Name the hormones produced only during pregnancy in a human female. Mention their source organ.
→
List the disadvantages of insulin obtained from the pancreas of slaughtered cows and pigs:
→
Digrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli ?
→
How BOD is related to water pollution?
→
  1. ‘‘India has greater ecosystem diversity than Norway.’’ Do you agree with the statement? Give reasons in support of your answer.
  2. Write the difference between genetic biodiversity and species biodiversity that exists at all the levels of biological organisation.
→
  1. One of the codons on mRNA IS AUG. Draw the structure of RNA adapter molecule for this codon.
  2. Name the RNA polymerase that transcribes RNA in eukaryotes.
  3. What is unique about the amino acid this RNA binds with?
→
List six advantages of ex-situ approach to conservation of biodiversity.
→
A number of passengers were severely burnt beyond recognition during a train accident. Name and describe a modern technique that can help hand over the dead to their relatives.

OR

Following the collision of two trains a large number of passengers are killed. A majority of them are beyond recognition. Authorities want to hand over the dead to their relatives. Name a modern scientific method and write the procedure that would help in the identification of kinship.

→
What are bacteriophage vectors? Name the two phage vectors that are commonly used.
→
What type of organs eye of an Octopus and that of a human called? Give another example from the animal group and one from the plants of such organs. Name and explain the evolutionary process they exhibit.
→