Question
- Why must bacterial cells be first made 'competent' in rDNA technology? How is the process carried out?
- Name the methods by which an alien DNA can be made to enter:
- A plant cell.
- An animal cell.
✓
Answer
- DNA is a hydrophilic molecule that cannot pass through cell membrane; so the bacterial cells must be made competent to take up the DNA.
- The bacterial cells are made competent by treating them with a specific concentration of a divalent cation, such as calcium; this increases the efficiency with which DNA enters the bacterium.
- Biolistics/ Gene gun.
- Micro-injection.
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