Write any four ways used to introduce a desired DNA segment into … - Vidyadip

Question

Write any four ways used to introduce a desired DNA segment into a bacterial cell in recombinant technology experiments.

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Answer

Recombinant DNA technology involves the steps are:
Restriction enzymes, polymerare enzymes, ligases, vectors, and the host organisms.

Isolation of DNA.
Fragmentation of DNA by restriction endonucleases.
Isolation of the desired DNA fragment.
Amplification of the gene of interest.
Ligation of the DNA fragment into a vector using DNA ligase.
Transfer of becombinant DNA into the host.
Culturing the host cells on a suitable medium on a large scale.
Extraction of the desired product.
Downstream processing of the product as a finished product ready for marketing.

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