Who revealed biochemical nature of transforming principle? How was it done?
Answer
Prior to the work of Osward Avery, Colin Macleod and Maclyn McCarty (1933- 44), the genetic material was though to be protein. They worked to determine the biochemical nature of 'transforming principle' in Griffith's experiment. $\rightarrow$ The purified biochemical (proteins, DNA, RNA etc.) from the heat killed scells to see which ones could transform live R cells into cells. $\rightarrow$ They discovered that DNA alone from S bacteria caused R bacteria to become transformed. $\rightarrow$ They also discovered that protein - digesting enzymes (proteases) and RNA digesting enzymes (RNAases) did not affect transformation, so the transforming substance was not a protein or RNA. $\rightarrow$ Digestion with DNAase did inhibit transformation, suggesting that the DNA caused the transformations. $\rightarrow$ They calculated that DNA is the hereditary material.
The procedure of GIFT involves the transfer of female gamete to the fallopia tube can gametes be transferred to the uterus to achieve the same result? Explain
Answer
The uterine environment in not cogenial for the survivial of the gamete. If, directly transferred to the uterus they will undergo degeneration or could be phagocytosed and hence viable zygote would not be formed.
In an artificial hybridisations programme observed events as follows. Rearrange them in collect series as per hysridisation programme. (a) Re bagging (b) Choose parents (c) Bagging (d) spread pollen on stigma (e) Emasculation (f) collect pollen from male parental plant
Answer
Correct sequential order of an artificial hybridization is as follows : (b) selection of parents (e) emasculation (c) bagging (f) collection of pollen from male percent (d) dusting the pollen on stigma and (a) re-bagging.