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Question 15 Marks
Describe incomplete dominance exhibited by Mirabilis jalapa.
Answer
The German Botanist Carl Correns's (1905) Experiment - In 4 O' clock plant, Mirabilis jalapa when the pure breeding homozygous red $\left(R^1 R^1\right)$ parent is crossed with homozygous white $\left(R^2 R^2\right)$, the phenotype of the $F_1$ hybrid is heterozygous pink $\left(R^1 R^2\right)$. The $F_1$ heterozygous phenotype differs from both the parental homozygous phenotype. This cross did not exhibit the character of the dominant parent but an intermediate colour pink. When one allele is not completely dominant to another allele it shows incomplete dominance. Such allelic interaction is known as incomplete dominance. $F _1$ generation produces intermediate phenotype pink coloured flower.

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When pink coloured plants of $F_1$ generation were interbred in $F_2$ both phenotypic and genotypic ratios were found to be identical as $1: 2: 1$ (1 red: 2 pink: 1 white). Genotypic ratio is $1 R^1 R^1: 2$ $R^1 R^2: 1 R^2 R^2$. From this we conclude that the alleles themselves remain discrete and unaltered proving the Mendel's Law of Segregation. The phenotypic and genotypic ratios are the same. There is no blending of genes. In the $F^1$ generation $R^1$ and $R^2$ genes segregate and recombine to produce red, pink and white in the ratio of $1: 2: 1 . R^1$ allele codes for an enzyme responsible for the formation of red pigment. $R ^2$ allele codes for defective enzyme.

$R^1$ and $R^2$ genotypes produce only enough red pigments to make the flower pink. Two $R^1 R^2$ are needed for producing red flowers. Two $R^2 R^2$ genes are needed for white flowers. If blending had taken place, the original pure traits would not have appeared and all $F_2$ plants would have pink flowers. It is very clear that Mendel's particulate inheritance takes place in this cross which is confirmed by the reappearance of original phenotype in $F _2$.
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Question 25 Marks
How does the wrinkled gene make Mendel’s peas wrinkled? Find out the molecular explanation.
Answer
The protein called starch branching enzyme (SBEI) is encoded by the wild-type allele of the gene (RR) which is dominant. When the seed matures, this enzyme SBEI catalyzes the formation of highly branched starch molecules. Normal gene (R) has become interrupted by the insertion of extra piece of DNA (0.8 kb) into the gene, resulting in allele. In the homozygous mutant form of the gene (R) which is recessive, the activity of the enzyme SBEI is lost resulting in wrinkled peas.

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The wrinkled seed accumulates more sucrose and high water content. Hence Ore osmotic pressure inside the seed rises. As a result, the seed absorbs more water and when it matures it loses water as it dries. So it becomes wrinkled at maturation. When the seed has at least one copy of normal dominant gene heterozygous, the dominant allele helps to synthesize starch, amylopectin an insoluble carbohydrate, with the osmotic balance which minimises the loss of water resulting in smooth structured round seed.
 
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Question 35 Marks
Explain Dihybrid cross in pea plant.
Answer
solution The crossing of two plants differing in two pairs of contrasting traits is called dihybrid cross. In dihybrid cross, two characters (colour and shape) are considered at a time. Mendel considered the seed shape (round and wrinkled) and cotyledon colour (yellow \& green) as the two characters. In seed shape round $(R)$ is dominant over wrinkled $(r)$; in cotyledon colour yellow $(Y)$ is dominant over green (y).
Hence the pure breeding round yellow parent is represented by the genotype RRYY and the pure breeding green wrinkled parent is represented by the genotype rryy. During gamete formation the paired genes of a character assort out ' independently of the other pair. During thus will be genetically different and they are of the following four types:

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(1) Yellow round (YR) – 9/16
(2) Yellow wrinkled (Yr) – 3/16
(3) Green round (yR) – 3/16
(4) Green wrinkled (yr) -1/16


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These four types of gametes of $F_1$ dihybrids unite randomly in the process of fertilization and produce sixteen types of individuals in $F_2$ in the ratio of 9:3:3:1 as shown in the figure. Mendel's 9:3:3:1 dihybrid ratio is an ideal ratio based on the probability including segregation, independent assortment and random fertilization. In sexually reproducing organism / plants from the garden peas to human beings, Mendel's findings laid the foundation for understanding inheritance and revolutionized the field of biology. The dihybrid cross and its result led Mendel to propose a second set of generalisations that we called Mendel's Law of independent assortment.
 
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[ 5 Marks Questions ] - BIO BOTANY STD 12 Questions - Vidyadip