Question
Explain different types of restriction enzymes?
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Answer
Different types of restrictions enzymes are as follows:
- Type I – They function . simultaneously as endonuclease and methylase e.g. EcoK.
- Type II – They exhibit separate cleaving and methylation activities. They are more stable and are used in r-DNA technology e.g. EcoRI, Bgll. They cut DNA at specific sites within the pallindrome. Thousands of type II restriction enzymes have been discovered.
- Type III – They cut DNA at specific non- palindromic sequences e.g. Hpal, MboII.
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