1. Carbon dioxide (CO2)
2. Test Tube A had no yeast to perform fermentation, and Test Tube C had no sugar as food for yeast, so no gas was produced.
3. CO2 reacted with lime water (calcium hydroxide) to form calcium carbonate, a white precipitate, making it milky.
4. Fermentation
5. Sugar is essential as an energy source for yeast to carry out fermentation and produce CO2.