Question
Microbes can be used to decrease the use of chemical fertilizers and pesticides. Explain how this can be accomplished.###How biofertilizers increase fertility of soil?

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Nematode specific genes were introduced into the tobacco host plant using a vector:
  1. pBR 322.
  2. Plasmid.
  3. Bacteriophage.
  4. Agrobacterium.
A regulatory body working under MoEF for the release of transgenic crop is ____________:
  1. NBPGR
  2. NSC
  3. NIPGR
  4. GEAC
Read the following and answer any four questions from (i) to (v) given below:

Discovery of penicillin by Alexander Fleming in 1928 marked the beginning of the remarkable era in medical field. Penicillin was the first antibiotic extracted from Penicillium notatum. Antibiotics are used to treat bacterial diseases. These can be broad spectrum which can kill diverse group of disease causing bacteria and narrow spectrum which is effective only against one group of pathogenic strain. Antibiotics can act as bactericides or bacteriostatic. Bactericidal antibiotics kill bacteria by - disruption of cell wall synthesis (e.g., penicillin, cephalosporin, etc.), inhibition of SOS ribosome function (e.g.; erythromycin), inhibition of 30S ribosome function (e.g., streptomycin, neomycin), inhibition of amino acid-tRNA binding to ribosome (e.g., tetracyline). etc. Bacteriostatic antibiotics do not kill the bacteria rather they restrict the growth of bacteria. Penicillin belongs to $\beta$-lactum group of antibiotics and it inhibits bacterial cell wall synthesis by binding and inactivating protein. It inhibits transpeptidation of reaction and blocks cross-linking of the cell wall. It is used to treat tonsilitis, sore throat, gonorrhoea, rheumatic fever and some pneumonia types.

  1. The first antibiotic was extracted from a:
  1. Lichen.
  2. Fungus.
  3. Eubacteria.
  4. Acti-nomycetes.
  1. Which of the following kills bacteria by interfering SOS ribosome function?
  1. Cephalosporin.
  2. Erythromycin.
  3. Streptomycin.
  4. Neomycin.
  1. $\beta$-lactum group of antibiotics kill the bacterial pathogen by:
  1. Disruption of plasma membrane.
  2. Inhibition of translation of mRNA.
  3. Inhibition of translation of mRNA
  4. Inhibition of transcription of mRNA.
  1. Penicillin is not used to treat.
  1. Pneumonia.
  2. Tonsilitis.
  3. Rheumatic fever.
  4. Candidiasis.
  1. Assertion: Cephalosporins act by disruption of bacterial cell wall synthesis mechanism.

Reason: Cephalosporins are bacteriostatic antibiotics.

  1. Both assertion and reason are true and reason is the correct explanation of assertion.
  2. Both assertion and reason are true but reason is not the correct explanation of assertion.
  3. Assertion is true but reason is false.
  4. Both assertion and reason are false.
Read the following and answer any four questions from (i) to (v) given below:
IUCN maintains a Red Data Book or Red List which is a catalogue of taxa facing risk of extinction. The IUCN Red List (2004) documents the extinction of 784 species in the last 500 years. Some examples of recent extinctions include the dodo, quagga, thylacine and Steller's sea cow. The last twenty years alone have witnessed the disappearance of 27 species. Red List has eight categories of species.
  1. Dodo, an extinct taxon, belongs to which country?
  1. Mauritius
  2. Africa
  3. Australia
  4. Russia
  1. To which of the following categories of IUCN, Berberis nilghiriensis belongs?
  1. Extinct.
  2. Extinct in wild.
  3. Endangered.
  4. Critically endangered.
  1. Steller's sea cow and passenger pigeon became extinct due to:
  1. Alien species invasion.
  2. Over-exploitation.
  3. Coextinctions.
  4. Intensive agriculture.
  1. Bali, Javan and Caspian are:
  1. Species of tiger.
  2. Species of cheetah.
  3. Subspecies of cheetah.
  4. Subspecies of tiger.
  1. Select the correct term for the following definitions (i, ii, iii, iv).
  • The taxon is liable to become extinct if not allowed to realise its full biotic potential by providing protection from exotic species/ human exploitation/habitat deterioration/depletion of food.
  • The taxon has been completely eliminated or died out from earth, e.g., Dodo.
  • The taxon is facing a high risk of extinction in the wild in the near future due to decrease in its habitat, excessive predation or poaching.
  • They are species with naturally small populations, either localised or thinly scattered, which are always at risk from pests/ pathogens/ predators/ exotic species.
 
(i)
(ii)
(iii)
(iv)
(a)
Threatened
Extinct
Endangered
Rare
(b)
Endangered
Extinct
Threatened
Rare
(c)
Extinct
Rare
Threatened
Endangered
(d)
Threatened
Extinct
Rare
Endangered
Read the following text carefully and answer the questions that follow:
Gene regulation is the mechanism of switching off and switching on of the genes depending upon the requirement of cells and the state of development. Gene regulation is of two types: negative and positive. In negative gene regulation, the genes continue expressing their effect till their activity is suppressed. Positive gene regulation is the one in which the genes remain non-expressed unless and until they are induced to do it. Operon model is a co-ordinated group of genes such as structural gene, operator gene, promoter gene, regulator gene which function together and regulate a metabolic pathway as a unit, e.g., lac operon, trp operon, ara operon, etc.
Image
i. Regulation of gene expression occurs at which the level? (1)
ii. What is complementary to an mRNA molecule transcribed from the lac operon contains nucleotide sequences? (1)
iii. Describes the control of transcription of the genes involved in the breakdown of lactose in Escherichia coli?
(2)
OR
What is the function of catabolic activator protein in lac operon? (2)
Assuming that within a population of beetles where Hardy Weinberg conditions are met, the colour black (B) is dominant over the colour red (b). 40% of all beetles are red (bb).
Given this information, answer the questions below:
A. What is the frequency of red beetles?
B. Calculate is the percentage of beetles in the population that are heterozygous.
C. What is the frequency of homozygous dominant individuals?
OR
D. Assuming that Hardy Wienberg conditions are met in the beetle population consisting of 1500 beetles. How many beetles would you expect to be black and red in colour respectively?
Microbes are useful in household products. Explain this with suitable examples
Read the following and answer any four questions from (i) to (v) given below:

Saurin, a M.Sc student, get an assignment on sewage treatment plant (STP) to study the microbial load. After visiting such plant in his locality, he makes a simplified diagram of the STP for his project. Study the diagram given below and answer the following questions.

  1. In the diagram 'A' denotes:
  1. Aeration tank.
  2. Primary settling tank.
  3. Secondary settling tank.
  4. Sludge digester.
  1. Which of the following is incorrect regarding the sludge released from A?
  1. It is formed after primary treatment.
  2. It does not require aeration.
  3. It possesses floes of decomposer microbes.
  4. It is used in landfills.
  1. A large number of aerobic heterotrophic microbes grow in:
  1. A
  2. B
  3. C
  4. Both (a) and (b).
  1. What is denoted by 'D' in the given diagram?
  1. Primary sludge.
  2. Primary effluent.
  3. Activated sludge.
  4. Secondary effluent.
  1. Assertion: The colloided and finely suspended matter of sewage form aggregates which are called floes.

Reason: Floes contain masses of bacteria, slime and fungal filaments.

  1. Both assertion and reason are true and reason is the correct explanation of assertion.
  2. Both assertion and reason are true but reason is not the correct explanation of assertion.
  3. Assertion is true but reason is false.
  4. Both assertion and reason are false.
Read the following text carefully and answer the questions that follow:
Malaria and dengue fever are major mosquito-borne public health problems in tropical countries. The authors report a malaria and dengue co-infection in an 11-year-old boy who presented with sustained fever for 10 days. The physical examination revealed a flushed face, injected conjunctivae and left submandibular lymphadenopathy. His peripheral blood smear showed few ring-form trophozoites of Plasmodium falciparum. His blood tests were positive for dengue NS-1 antigen and IgM antibody, and negative for IgG antibody. After the initiation of antimalarial treatment with artesunate and mefloquine, his clinical condition gradually improved. However, he still had low-grade fever that persisted for 6 days. Finally, he recovered well without fluid leakage, shock or severe bleeding.
i. Name the fish that help in eradication of mosquito larvae.
ii. What is the reason of symptoms of malaria?
iii. Name the body parts and host in which following events takes place in life cycle of plasmodium.
a. asexual reproduction
b. sexual reproduction.
OR
Name some vector borne diseases and their vector.
Give a brief account of evolution of vertebrates.