MCQ
Polyethylene glycol method is used for:
- ASeedless fruit production
- BEnergy production from sewage
- CGene transfer without a vector
- DBiodiesel production
Explanation:
Polyethylene glycol (PEG) is a polyether compound. The compound has varied applications. Its biological uses include being used as a precipitant for plasmid DNA isolation and protein crystallisation. PEG is also used as a fusing agent in the transformation experiments. The gene or the desired fragment of DNA can be transferred into the host cell without the use of vector. PEG with calcium ions allows the membrane to become charged and allow the entry of the gene.
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$(i)$ Bacillus thuringiensis are available in sachets as dried spores which are mixed with kerosine and sprayed on to vulnerable plants
$(ii)$ B. thuringiensis are used to control butterfly caterpillars, but leave other insects unharmed
$(iii) $ The toxin of B. thuringiensis is released in the blood of larvae and the larvae get killed
$(iv)$ By the development of methods of genetic engineering B. thuringiensis toxin genes have been introduced