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MODEL PAPER 10 — BIOLOGY STD 12 Science — Question

Rajasthan BoardEnglish MediumSTD 12 ScienceBIOLOGYMODEL PAPER 104 Marks
Question
Recombinant DNA (rDNA) is a technology that uses enzymes to cut and paste together DNA sequences of interest. The recombined DNA sequences can be placed into vehicles called vectors that ferry the DNA into a suitable host cell where it can be copied or expressed.
i. A bacterial cell is shown in the figure given below. Label the part 'A' and 'B'. Also, mention the use of part A in rDNA technology.
Image
ii. Suppose a linear DNA fragment and a plasmid has three restriction sites for EcoRI. How many fragments will be produced from linear DNA and plasmid, respectively?
✓

Answer

i. In the above bacterial cell, i.e. A is plasmid B is chromosomal DNA. The plasmid is used as a vector in rDNA technology.
ii. If the enzyme EcoRl acts on both linear DNA and plasmid DNA, each having three recognition sites, the restriction enzyme will generate 3 fragments from plasmid DNA (as it is circular) and 4 fragments from linear DNA.

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