Question
What is the cause of frameshift mutation?

Answer

Deletions and insenions of base pairs of DNA cause frameshift mutation.

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Distinguish between : Granulocytes and Agranulocytes.
Highlight five areas where biotechnology has influenced our lives.
Read the following and answer any four questions from $2(i)$ to $2(v)$ given below: Restriction endonuclease was isolated for the first ti me by W. Arb er in $1962$ in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called as molecular scissors or biological scissors. Three types of restriction endonucleases are Type I, Type II and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC in DNA duplex and cut strands between G and A.
  1. Only type II restriction enzymes are used in gene manipulation because
  1. ATP is not required for cleaving.
  2. It consists of three different subunits.
  3. It makes cleavage or cut in both the strands of DNA molecule.
  4. Both (a) and (c).
  1. Which of the following ions are used by restriction endonucleases for restriction?
  1. $Mg^{2+}$ ions
  2. $Mn^{2+}$​​​​​​​ ions
  3. $Na^{2+}$​​​​​​​ ions
  4. $K^{2+}$​​​​​​​ ions
  1. Restriction endonuclease was isolated for the first time in a:
  1. Plant cell.
  2. Animal cell.
  3. Prokaryotic cell.
  4. Genninal cell.
  1. Restriction endonucleases are also called as molecular or biological scissors because,
  1. They cleave base pairs of DNA only at their terminal ends.
  2. They cleave one or both the strands of DNA.
  3. They act only on single stranded DNA.
  4. None of these.
  1. Select the option that correctly states the working action of restriction endonuclease EcoR I on DNA sequence GAATTC
  1. $\ \ \ \ \ \ \ \ \ \ \ \ \downarrow\\5'-\text{GAATTC}-3'\\3'-\text{CTTAAG}-5'\\\ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \uparrow$
  2. $ \ \ \ \ \ \ \ \ \ \ \downarrow\\5'-\text{GAATTC}-3'\\3'-\text{CTTAAG}-5'\\\ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \uparrow$
  3. $\ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \downarrow\\5'-\text{GAATTC}-3'\\3'-\text{CTTAAG}-5'\\\ \ \ \ \ \ \ \ \ \uparrow$
  4. $\ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \downarrow\\5'-\text{GAATTC}-3'\\3'-\text{CTTAAG}-5'\\\ \ \ \ \ \ \ \ \ \ \ \ \ \ \uparrow$
Distinguish between : Atria and Ventricles.
Read the following and answer any four questions from (i) to (v) given below:
A 23 year old Sahil has been diagnosed with an infection of reproductive tract caused by bacteria. He complains about burning sensation during urination, pus-containing discharge and pain around genitalia. This infection has incubation period of 2-5 days but can be cured.
  1. From which disease is Sahil suffering?
  1. Chlamydiasis.
  2. Herpes.
  3. Gonorrhoea.
  4. Syphilis.
  1. Which among the following reproductive tract infections is transmitted by bacteria?
  1. Trichomoniasis.
  2. Chancroid.
  3. Genital warts.
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  1. Which mode of treatment would be useful for Sahil?
  1. Cryosurgery.
  2. Use of Podophyllum preparation.
  3. Use of antibiotic ampicillin.
  4. It cannot be treated.
  1. What technique was used to diagnose Sahil's disease?
  1. Gram staining of discharge and culture.
  2. ELISA Test.
  3. Antibody detection.
  4. Antigen test.
  1. Find the odd one out.
  1. Treponema pallidum.
  2. Neisseria gonorrhoeae.
  3. Haernophilus ducreyi.
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Ectomycorrhizae and Endomycorrhizae.
Distinguish between: Allopatric and Sympatric speciation.
Read the following and answer any four questions from (i) to (v) given below:
The endosperm makes the main source of food for the embryo. Generally the endosperm nucleus divides after the division of the oospore, but in several cases the endosperm is formed to a great extent even before the first division of the oospore. There are three general types of endosperm formation: (a) nuclear type, (b) cellular type and (c) helobial type. The endosperm is usually triploid, but haploid endosperm is also found. Endosperm may either be completely consumed by the developing embryo before seed maturation, or it may persist in the mature seed.
  1. Haploid endosperm is found in:
  1. Pin us.
  2. Cauliflower.
  3. Sunflower.
  4. Pea.
  1. Persistent endosperm is found in:
P. Pea Q. Castor R. Bean S. Coconut T. Groundnut.
  1. Q and S.
  2. P and T.
  3. R, S and T.
  4. P, S and T.
  1. Milk of tender coconut represents (A) and the surrounding white coconut meal represents (B).
S. No
A
B
(a)
Cellular endosperm
Free- nuclear endosperm
(b)
Free-nuclear endosperm
Cellular endosperm
(c)
Helobial endosperm
Cellular endosperm
(d)
Free-nuclear endosperm
Helobial endosperm
  1. If an endosperm cell of a gymnosperm contains 12 chromosomes, the number of chromosomes in each cell of the root will be:
  1. 4
  2. 24
  3. 16
  4. 6
  1. In angiosperms, normally after fertilization:
  1. The zygote divides earlier than the primary endosperm nucleus.
  2. The primary endosperm nucleus divides earlier than the zygote.
  3. Both the zygote and primary endosperm nucleus divide simultaneously.
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Read the following and answer any four questions from (i) to (v) given below: The mature ovum or a female gamete is spherical in shape. The human ovum is almost free of yolk and is said to be alecithal. Human ovum loses its ability to be fertilised about 24 hours after ovulation. Refer to the given structure of ovum and answer the following questions.
  1. Thick cellular layer formed of radially elongated follicular cells is:
  1. Zona pellucida.
  2. Plasma membrane.
  3. Perivitelline membrane.
  4. Corona radiata.
  1. In humans, at which stage does ovum get released from ovary?
  1. Secondary oocyte.
  2. Oogonium.
  3. Primary oocyte.
  4. First polar body.
  1. Cytoplasm of an ovum is enveloped by _____.
  1. Zona pellucida.
  2. Corona radiata.
  3. Cell membrane.
  4. Perivitelline space.
  1. Select the correct option:
S. No
V
W
X
(a)
Cytoplasm
Zona pellucida
Plasma membrane
(b)
Cortical granules
Corona radiata
Zona pellucida
(c)
Cortical granules
Plasma membrane
Corona radiata
(d)
Cytoplasm
Corona radiata
Zona pellucida
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  2. Only one ovum formed from one oogonium.
  3. It lacks centrioles.
  4. It has very small amount of ooplasm.